Separation and Purification Strategies for Biotechnology Products is an introductory course that helps participants understand and develop strategies for separating and purifying proteins, recombinant DNA products, and monoclonal antibodies. Hands-on exercises include membrane filtration, chromatography techniques, and column packing. The exercises allow participants to integrate theory with practice and to understand practical issues in purification of biotechnology products. Lectures provide background that helps participants understand the theory and principles of bioseparations.

Approximately 75% of the time is spent in lecture and 25% is in the laboratory.

"Great training. Even with a non-Chemistry background I was able to learn the concepts behind the different separation and purification strategies that will enable me to have a broader view of the process I support."
Victor M. Cruz, MI & CS Manager, Lilly del Caribe, Incorporated

Day 1
(Registration begins at 8:00 a.m.)

• Lecture
  • Fundamentals of separation techniques, centrifugation
  • Theory and principles of cell disruption
  • Reverse phase and chiral chromatography
  

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Hands-on Laboratory
• Centrifugation

Day 2

Lecture
• Gel filtration, affinity, ion exchange, hydrophobic interaction chromatography
• Developing purification strategies

Day 3

Lecture
• Optimization
• Scale-up considerations
• Downstream purification processing
• Process hygiene and column maintenance

Hands-on Laboratory
• Gel filtration optimization (group separation)
• Anion exchange chromatography

Day 4

Lecture
• Membrane characteristics
• Filtration set-up
• Crossflow filtration operation
• Process development and optimization

Hands-on Laboratory
• Dialfiltration/concentration experiments

Day 5
(Adjourns at Noon)

Lecture
• Hydrophobic interaction chromatography

Hands-on Laboratory
• Column packing

 

 

Faculty

Patrick Cirino, Ph.D., Penn State, Department of Chemical Engineering
Richard Henry, Ph.D., Consultant
Fast Trak Group Members, GE Healthcare
J. Kevin O’Donnell, Ph.D., Tosoh Bioscience, LLC
Beatrice Sirakaya, M.S., Penn State, Department of Biochemistry and Molecular Biology
Andy Steppe, Westfalia Separator, Inc.
Gregory Ziegler, Ph.D., Penn State, Department of Food Science

CLICK HERE TO
REGISTER ONLINE

Early Registration $2195

Regular Registration $2395
(within 4 weeks of course start date)

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