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Separation and Purification Strategies for Biotechnology Products is an introductory course that helps participants understand and develop strategies for separating and purifying proteins, recombinant DNA products, and monoclonal antibodies. Hands-on exercises include membrane filtration, chromatography techniques, and column packing.
The exercises allow participants to integrate theory with practice and to understand practical issues in purification of biotechnology products. Lectures provide background that helps participants understand the theory and principles of bioseparations.
Approximately 75% of the time is spent in lecture and 25% is in the laboratory.
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Lecture:
• Fundamentals of separation techniques, centrifugation
• Theory and principles of cell disruption
• Reverse phase and chiral chromatography
Laboratory demonstration:
• Centrifugation
Lecture:
• Gel filtration, affinity, ion exchange, hydrophobic interaction chromatography
• Developing purification strategies
Lecture:
• Optimization
• Scale-up considerations
• Downstream purification processing
• Process hygiene and column maintenance
Hands-on Laboratory:
• Gel filtration optimization (group separation)
• Anion exchange chromatography
Lecture:
• Membrane characteristics
• Filtration set-up
• Crossflow filtration operation
• Process development and optimization
Hands-on Laboratory:
• Dialfiltration/concentration experiments
 (adjourns
at noon)
Lecture:
• Hydrophobic interaction chromatography
Hands-on Laboratory:
• Column packing
Patrick Cirino, Ph.D., Penn State, Department of Chemical Engineering
Richard Henry, Ph.D., Penn State, Department of Chemistry
Fast Trak Group Members, GE Healthcare
J. Kevin O’Donnell, Ph.D., Tosoh Bioscience, LLC
Beatrice Sirakaya, M.S., Penn State, Department of Biochemistry and Molecular Biology
Andy Steppe, Westfalia Separator, Inc.
Gregory Ziegler, Ph.D., Penn State, Department of Food Science
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