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This course emphasizes the theoretical and practical aspects of laboratory microbial fermentation and scale-up. Participants will get hands-on experience setting up and monitoring fermentors, as well as conducting practical experiments that include the effects of medium components on cell density, mixing, aeration, and oxygen mass transfer in fermentation systems, analysis and control of key parameters for product optimization, and computer control of fermentation. The laboratory work will be supported by presentations and discussions on the fundamentals of microbial culture, aseptic techniques, kinetics of fermentation, recombinant microorganisms, scale-up strategies, downstream processing, economic considerations, regulatory aspects, and cGMP.
Approximately 70% of the time is spent in lecture and 30% is in the laboratory.
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Lecture:
• Kinetics of fermentation
• Design of bioreactors for downstream processing
• Computer control of fermentation
Hands-on Laboratory:
• Hands-on operation of lab scale fermentors
- fermentor preparation
- medium preparation
- probe calibration
- sterilization
Lecture:
• Aeration and oxygen mass transfer
• Theory and practice of DO and pH probes
• Microbial growth and bioprocessing
Hands-on Laboratory:
• Preparing fermentors for inoculation, probe calibration
Lecture:
• Plasmid stability
• Recombinant microorganisms and strain improvement
• Evaluation of oxygen transfer
Hands-on Laboratory:
• Evaluation of oxygen transfer coefficients and rates
 (adjourns
at noon)
Lecture:
• Media formulations and nutrient requirements
• cGMP, validation and regulatory aspects of bioprocess fermentation
Ali Demirci, Ph.D., Penn State, Department of Agricultural and Biological Engineering
John Enders, Ph.D., Delmont Laboratories
Vikram Gossain, Ph.D., New Brunswick Scientific Co., Inc.
Kathleen Harrison, Becton Dickinson Microbiology Systems
Michael McMurtrie, Broadley James Corporation
Allen Phillips, Ph.D., Penn State, Department of Biochemistry and Molecular Biology
Ronald Porter, Ph.D., Penn State, Department of Biochemistry and Molecular Biology
Beatrice Sirakaya, M.S., Penn State, Department of Biochemistry and Molecular Biology
Song Tan, Ph.D., Penn State, Department of Biochemistry and Molecular Biology
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