This introductory course emphasizes cell culture and scale-up theory, as well as an introduction to laboratory techniques utilized in animal cell culture. These include modern techniques used with mammalian cell culture from frozen stocks to inoculation and cultivation in bench top bioreactors. Lectures provide the background to understand the principles of cell culture and bioreactor design. Participants will gain experience with a variety of bioreactor systems, including stirred tank bioreactors, microcarrier and hollow fiber systems. The course covers mammalian and insect cell culture systems and is highly recommended for those who want to learn cell line propagation, characterization, and quality control, and be introduced to the complex issues of scale-up of cell culture from the research bench to larger reactors.

Approximately 50% of the time is spent in lecture and 50% is in the laboratory.

"Great educational and hands-on program."
Nihal Behrens, Biotechnology Professor, Ellsworth Community College

Day 1
(Registration begins at 8:00 a.m.)

Lecture
• Mammalian cells in culture
• Medium formulations and serum-free medium
• Primary and continuous cell lines

Hands-on Laboratory
• Cell growth and subculturing methods
• Primary cell culture from chicken embryo
• Establishing a primary cell line

Day 2

Lecture
• Cell line characterization for production of biologics
• Monoclonal antibody production
• Transformation of mammalian cells with SV40 DNA

Hands-on Laboratory
• Mycoplasma detection
• Fusion of cells in monolayer
• Cell transformation with viral DNA via transfection assay

Day 3

Lecture
• Validation, cGMP and change control
• ELISA theory
• Design and operation of stirred-tank bioreactors for animal cell culture

Hands-on Laboratory
• ELISA techniques
• Stirred tank reactor design and instrumentation, pH calibration, reactor preparation and sterilization
• DO and pH probes, probe maintenance, and calibration

Day 4

Lecture
• Achieving precision with pH measurements and dissolved oxygen
• Perfusion techniques in stirred tank bioreactors

Hands-on Laboratory
• Growth of anchorage-dependent cells on microcarriers in stirred tank reactors
• Growth of suspension cultures in bioreactors
• Medium addition to bioreactors, cell counts, viability, seeding reactors

Day 5
(Adjourns at Noon)

Lecture
• Insect cell culture
• Scale-up strategies for insect cell culture

Hands-on Laboratory
• Insect cell counting
• Plaque assays

Faculty

John Enders, Ph.D., Delmont Laboratories
Richard Frisque, Ph.D., Penn State, Department of Biochemistry and Molecular Biology
Glenn P. Godwin, Massachusetts Biologic Laboratories
Vikram Gossain, Ph.D., New Brunswick
Robert Lumley-Sapanski, Penn State, Department of Food Science
Andrea Mastro, Ph.D., Penn State, Department of Biochemistry and Molecular Biology
Michael McMurtrie, Broadley James Corporation
Dennis Powell, Centocor, Inc.
Beatrice Sirakaya, M.S., Penn State, Department of Biochemistry and Molecular Biology

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Early Registration $2195

Regular Registration $2395
(within 4 weeks of course start date)

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